These mutants of Klentaq DNA Polymerase are designed for "Hot Start" PCR due to their suppressed activity at room temperature. At last a "Hot Start" can be achieved without any changes to the protocol.
New "Cesium Taq" Cold-Sensitive Polymerase
from DNA Polymerase Technology, Inc.
U.S. Patent number 6,214,557. Not licensed for PCR
No more must you cook your reactions at 95 degrees first, or manually add reaction components on the run at the thermal cycler, or use the highly priced blocking antibodies or chemically modified Taq polymerases.
A hot start is beneficial or necessary for specificity, efficiency and reliability of amplification of a large proportion of the most demanding of PCR reactions. A heat-switch can be achieved in PCR protocols by several methods, all of which have drawbacks such as narrow conditions, prone to error or contaminations, or extra expense, and/or lack of applicability to long PCR.
As an alternative to those methods,
we have isolated cold-sensitive mutants of Klentaq DNA Polymerase.
They are heat-switchable, and they can still do PCR. These mutants
are less active than the wild-type at low (reaction set up) temperatures,
yet normally active at 65-70 degrees, and still resistant to 95 degrees.
The novel mutant enzymes series, called Cs-Taq, can outperform the wild-type
enzyme for targets which benefit from a hot start. (See
data with CsTaq). At present, the mutants are in a background
of Klentaq1 (an N-terminal, robust deletion of Taq DNA polymerase which
lacks 5'-exonuclease and provides higher yields of product). We are
also working on developing analogous mutants of full-length Taq DNA polymerase.
PCR troubles? Give your tough amplification reactions an extra chance.Beta-test samples are not initially free, but they qualify for an additional single free aliquot if you send back some data such as a gel picture. This gel should compare Cs-Taq to some other method of providing a hot start; ideally, they should include control reactions without a hot start (i.e. wild-type Klentaq1 or wild-type Taq, set up at room temperature). Our publication is in preparation, and when it comes out you will be able to see exactly what mutation(s) you have. If you need this information sooner, it can be provided under a confidentiality agreement. Beta testers will get a preprint, and be the first to be notified of new mutants as they come out.
Contact us (orders@klentaq.com or (314) 771-5566) to beta-test Cesium Taq.
Currently, there is no extra charge compared to wild-type Klentaq1.
We appreciate your feedback and comments.
