Our Hot Start enzymes with aptamers and their standard counterparts were used to amplify a 300 bp human gene target from 10 ng purified DNA. Reactions were 25 ul and 0.125 ul of each enzyme was used. PCR reactions were performed in duplicate. A competitor Hot Start Taq was also included. The PCR mix were stored at 4°C or incubated at 25°C for 1 hour prior to PCR. *A555 is one of our in-progress enzymes. Results: specific high-yield products were achieved with all of our Hot Start enzymes, and primer dimers were significantly reduced compared to the standard enzymes. All of our Hot Start enzymes outperform the Hot Start competitor Taq.
Our full length Taq mutant Hot Start enzymes with aptamers and their standard counterparts were used to amplify a 300 bp human gene targe from 5% EDTA treated human blood in the absence of any PCR Enhancer Cocktail.. Reactions were 25 ul and 0.25 ul of each enzyme was used. PCR reactions were performed in duplicate. A competitor Hot Start Taq was also included. The PCR mix were stored at 4°C or incubated at 25°C for 1 hour prior to PCR. *A555 is one of our in-progress enzymes. Results: specific high-yield products were achieved with Hot Start OmniTaq 2 and Hot Start OmniTaq 3. Neither version of OmniTaq, CesiumTaq, or A555 were able to amplify this target from blood under this stringent condition. The Hot Start Competitor also failed.
Our truncated Hot Start Klentaq mutant enzymes with aptamers and their standard counterparts were used to amplified a 300 bp human gene targe from 5% EDTA treated human blood in the absence of any PCR Enhancer Cocktail.. Reactions were 25 ul and 0.25 ul of each enzyme was used. PCR reactions were performed in duplicate. A competitor Hot Start Taq was also included. The PCR mix were stored at 4°C or incubated at 25°C for 1 hour prior to PCR. *A555 is one of our in-progress enzymes. Results: specific high-yield products were achieved with all of our truncated Hot Start Klentaq mutant enzymes, and primer dimers were significantly reduced compared to the standard enzymes. Both the Hot Start Taq Competitor and Standard Taq Competitor failed to amplify this target from blood.