Klentaq-LA
Klentaq-LA is for Long and Accurate PCR. It is almost the original mixture of enzymes that was the invention of Long PCR (Barnes, 1994). Klentaq-LA also has inhibition-resistance properties, tolerating up to 10% whole blood or serum in a PCR reaction.
8-20 kb should be so easy and reliable with Klentaq-LA that we will refund a tube of your purchase if it doesn't work for you, if you show us the data. 35 kb is attainable with more practice, at least from phage or cosmid template. Longer PCR amplicons require more excellent and intact preparation of the template DNA. KlentaqLA gives more reliable and efficient PCR at all sizes of amplicon, down even below 1 kb. Our original-strength enzyme is twice the concentration of licensed KlentaqLA from other providers, but theirs works well if you put in twice as much, and use our buffer.
100 ul of Klentaq-LA is sufficient for 1000 50 ul reactions of 1 kb length. For each kb in amplicon length, add 0.05 ul enzyme, not to exceed 0.65 ul per 50 ul reaction. For instance, for a 4 kb product, add 0.2 ul enzyme for each 50 ul reaction. For a 30 kb product, add 0.65 ul per 50 ul reaction.
To accurately pipet 0.05 ul for a typical 50 ul PCR of 1 kb or less, make up 1 ml of master mix and measure 1 ul of KlentaqLA into the whole ml. This mastermix will allow you to test several variables, such as template concentration, annealing temperature, PCR enhancers, etc.
Klentaq1, the non-LA version (for short and easy targets, or if the proof-reading function is NOT desired.)