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Frequently Asked Questions

… the prefix d is added, such as dATP. Dideoxy means two positions with no OH group (the 2' and 3' positions). Perkin Elmer has recently raised the price (by …

Our Publications

… Procedure: Live culture-based qPCR screening of Taq DNA polymerase variants for resistance to PCR inhibitors. … 13:1624725 OmniTaq 2:  A Single Amino Acid Change to Taq DNA Polymerase Enables Faster PCR, Reverse Transcription and …

PCR Enhancer Cocktail 1

… or EDTA, or plasma treated with heparin.  Please refer to our  PEC Comparison Page , for more information and for help … the right PCR Enhancer Cocktail for your application. Our family of PEC products are non-betaine based PCR … purification . They are highly recommended for use with our inhibition-resistant Taq and Klentaq mutants. When used …

Product Overview

… Klentaq1 and Klentaq LA Our 5'-exonuclease deficient Taq polymerase provides improved fidelity and thermostability, … are double cold-sensitive mutants of Taq or Klentaq1 DNA Polymerases.  They provide an automatic hot start for PCR … is a buffer system providing a hot-start for PCR with any DNA polymerase.  Hot Start Products PCR Enhancer Cocktails …

Hot Start Klentaq1

… patent 5,436,149). Klentaq1 also has inhibition resistance properties, tolerating 30 - 40% whole blood in a PCR … polymerase at sub-cycling temperatures, inactivating the enzyme and preventing spurious amplification.      … purified DNA.  Reactions were 25 ul and 0.125 ul of each enzyme was used.  PCR reactions were performed in duplicate. …