During standard PCR cycling, primer-dimer formation and other nonspecific primer annealing can occur while the machine is heating up. This can be detrimental for tough PCR reactions. It is possible to perform a manual hot-start by adding the enzyme or other critical reaction component after the tubes reach melting temperature. But using a hot start product makes the process much easier.
Our Cesium enzymes are double cold-sensitive mutants of Taq or Klentaq1 DNA Polymerases. They provide an automatic hot-start for PCR due to suppressed activity at low temperatures.
In addition, all enzymes listed are reversibly bound to an aptamer. The aptamer forms a hairpin and binds to the active site of the polymerase at sub-cycling temperatures, inactivating the enzyme and preventing spurious amplification. The aptamer is released at the first melt step, allowing full enzyme activity.
RockStart buffer is designed to provide a hot-start with any DNA polymerase. It works by preciptating the Magnesium at room temperature and preventing enzyme activity. The Magnesium is released upon normal cycling conditions.
| Product Name | Cat # | Amount | Price | Purchase | CITATIONS |
|---|---|---|---|---|---|
| Hot Start Klentaq1 | HS100 | 100 ul (2,000 X 25 ul rxns up to 1kb) | $305.00 | ||
| Hot Start OmniTaq | HS300 | 125 ul (500 x 25 ul rxns) | $320.00 | ||
| Hot Start OmniTaq 2 | HS302 | 125 ul (500 X 25 ul rxns) | $400.00 | ||
| Hot Start OmniTaq 3 | HS303 | 125 ul (500 X 25 ul rxns) | $400.00 | ||
| Hot Start ZipTaq | HS304 | 125 ul (500 X 25 ul rxns) | $400.00 | ||
| Hot Start Omni Klentaq | HS340 | 125 ul (500 X 25 ul rxns) | $320.00 | ||
| Hot Start Omni Klentaq 2 | HS342 | 125 ul (500 X 25 ul rxns) | $400.00 | ||
| Hot Start Cesium Klentaq C | HS280 | 100ul (2000 x 25ul rxns) | $250.00 | ||
| Hot Start Cesium Klentaq AC | HS240 | 100ul (2000 x 25ul rxns) | $250.00 | ||
| CesiumTaq | 200 | 100 ul (2,000 X 25 ul rxns) | $220.00 | ||
| Hot Start CesiumTaq | HS200 | 100 ul (2,000 X 25 ul rxns) | $250.00 | ||
| Hot Start Klentaq-S | HS105 | 100 ul (2,000 X 25 ul rxns up to 1kb) | $315.00 | ||
| RockStart Buffer System | Rock100 | 100 rxns | $60.00 |