Hot Start Klentaq1 is made with aptamer-based technology, enabling room temperature reaction set-up.
Klentaq1 is named after Klenow fragment, and it is a similar N-terminal deletion (removing the 5'-[flap]exonuclease of Taq DNA polymerase). PCR tests show that Klentaq1 has improved fidelity (about 25%) and thermostability (about 2 degrees) relative to wild-type Taq, and it gives higher yields of amplicon. We speculate that the higher yields arise because at the end stages of a PCR reaction, the 5'-exonuclease can begin to attack the PCR product, but we are open to another opinion. Klentaq1 was used in the original formula for discovery of Long and Accurate PCR (Barnes, 1994; US patent 5,436,149). Klentaq1 also has inhibition resistance properties, tolerating 30 - 40% whole blood in a PCR reaction.
Since Klentaq1 lacks the 5 prime exonuclease activity, it is not suitable for TaqMan assays.
The aptamer binds to the polymerase at sub-cycling temperatures, inactivating the enzyme and preventing spurious amplification.
Hot Start Klentaq Outperforms Klentaq1 and Hot Start Competitor
Hot Start Klentaq with aptamer and its standard counterparts was used to amplify a 300 bp human gene target from 10 ng purified DNA. Reactions were 25 ul and 0.125 ul of each enzyme was used. PCR reactions were performed in duplicate. A competitor Hot Start Taq was also included. The PCR reactions were stored at 4°C or incubated at 25°C for 1 hour prior to PCR. Results: Hot Start Klentaq provided a specific high-yield product, outperforming the Hot Start Taq competitor and standard Klentaq1.
See more data on our Performance Data page.