Hot Start Cesium Klentaq C LA is made with aptamer-based technology, enabling room temperature reaction set-up.
Cesium Klentaq C is a cold-sensitive mutant of Klentaq1 (5'-exonuclease deficient Taq polymerase with improved fidelity and thermostability). Due to its suppressed activity at low temperatures this enzyme is designed for hot start PCR performance. Addition of the aptamer further enhances its hot start performance. The aptamer binds to the polymerase at sub-cycling temperatures, inactivating the enzyme and preventing spurious amplification. The Long-Accurate feature allows for amplification of longer products with higher fidelity and accuracy. LA enzymes are not recommended for use with dUTP.
10x buffer composition is: 500 mM Tris-Cl pH 9.2, 160 mM ammonium sulfate, 0.25% Brij 58, and 35 mM magnesium chloride.