Hot Start OmniTaq 2 LA is made with aptamer-based technology, enabling room temperature reaction set-up.
OmniTaq 2 is a mutant of Taq DNA polymerase. This enzyme provides 2-3x faster PCR and some inhibition-resistance. The aptamer binds to the polymerase at sub-cycling temperatures, inactivating the enzyme and preventing spurious amplification. The Long-Accurate feature allows for amplification of longer products with higher fidelity and accuracy. LA enzymes are not recommended for use with dUTP.
10x buffer composition is: 500 mM Tris-Cl pH 9.1, 160 mM ammonium sulfate, 0.25% Brij 58, and 25 mM magnesium chloride.
Please note that Hot Start OmniTaq 2 LA is not yet optimized for use in RT-PCR or RT-LAMP.
We highly recommend pairing this product with one of our PCR Enhancer Cocktails.